14.8 Active B12

14.8.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 125.3961624 1 <0.0001 ***
Active.B12 1.6890917 1 0.2 ns
Study 0.0760030 1 0.8 ns
GA_samp 79.4814651 1 <0.0001 ***
Active.B12:Study 0.9626689 1 0.3 ns
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 25.1243667 1 <0.0001 ***
Active.B12 0.0656310 1 0.8 ns
Study 1.8324371 1 0.2 ns
GA_samp 1332.4521580 1 <0.0001 ***
Active.B12:Study 0.3863666 1 0.5 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 246.485079 1 <0.0001 ***
Active.B12 1.157844 1 0.3 ns
Study 1.217943 1 0.3 ns
GA_samp 621.522857 1 <0.0001 ***
Active.B12:Study 2.941978 1 0.09 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 1.768 1.768 0.2 ns
3 Study 1 Inf 0.626 0.626 0.4 ns
4 GA_samp 1 Inf 0.026 0.026 0.9 ns
2 Active.B12:Study 1 Inf 1.392 1.392 0.2 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 1.973 1.973 0.2 ns
3 Study 1 Inf 1.856 1.856 0.2 ns
4 GA_samp 1 Inf 0.046 0.046 0.8 ns
2 Active.B12:Study 1 Inf 1.963 1.963 0.2 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Active.B12 1 Inf 0.940 0.940 0.3 ns
3 Study 1 Inf 22.072 22.072 <0.0001 ***
4 GA_samp 1 Inf 1.408 1.408 0.2 ns
2 Active.B12:Study 1 Inf 0.006 0.006 0.9 ns

14.8.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 17.11 (10.56, 27.72) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.39
StudySTOP 0.88 (0.76, 1.01) 0.07
GA_samp 1.14 (1.11, 1.17) <0.0001
hPL (μg/ml)
Var Effect P
(Intercept) 1.90 (1.47, 2.44) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.76
StudySTOP 1.13 (1.05, 1.22) 0.002
GA_samp 1.34 (1.32, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.07 (0.05, 0.09) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.76
StudySTOP 0.97 (0.88, 1.08) 0.61
GA_samp 1.31 (1.28, 1.34) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 14.12 (7.58, 26.30) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.24
StudySTOP 0.74 (0.61, 0.89) 0.001
GA_samp 1.00 (0.97, 1.04) 0.83
HOMA-IR
Var Effect P
(Intercept) 3.78 (1.95, 7.33) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.22
StudySTOP 0.63 (0.51, 0.77) <0.0001
GA_samp 1.00 (0.96, 1.04) 0.88
Glucose (mmol/L)
Var Effect P
(Intercept) 5.91 (5.22, 6.69) <0.0001
Active.B12 1.00 (1.00, 1.00) 0.34
StudySTOP 0.85 (0.83, 0.88) <0.0001
GA_samp 0.99 (0.99, 1.00) 0.24

14.8.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Active B12 concentration (pM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 104.29589 8.572063 94.23585 88.59252 122.7827
STOP 91.66963 7.273354 81.94753 78.28460 107.3432
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 92.98739 2.718395 191.5457 87.77721 98.50682
STOP 104.92282 2.912509 153.5930 99.32399 110.83726
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.424099 0.0798185 358.0439 2.272102 2.586265
STOP 2.360972 0.0709449 275.7012 2.225361 2.504849
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 16.31124 1.0086717 Inf 14.44939 18.41300
STOP 12.00841 0.6038026 Inf 10.88142 13.25212
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.989104 0.2659184 Inf 3.500526 4.545875
STOP 2.499964 0.1335087 Inf 2.251521 2.775822
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.559648 0.0641395 Inf 5.435347 5.686791
STOP 4.738346 0.0534952 Inf 4.634649 4.844363

14.8.4 Comparisons

Effect for every 10 unit increase in Active B12 concentration (pM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) Active.B1271 / Active.B1261 SCOPE 1.01 (0.99, 1.02) 0.4
PRL (ng/ml) Active.B1271 / Active.B1261 STOP 1.01 (0.99, 1.02) 0.4
hPL (μg/ml) Active.B1271 / Active.B1261 SCOPE 1.00 (0.99, 1.01) 0.8
hPL (μg/ml) Active.B1271 / Active.B1261 STOP 1.00 (0.99, 1.01) 0.8
GH2 (ng/ml) Active.B1271 / Active.B1261 SCOPE 1.00 (0.99, 1.01) 0.8
GH2 (ng/ml) Active.B1271 / Active.B1261 STOP 1.00 (0.99, 1.01) 0.8
Insulin (uU/ml) Active.B1271 / Active.B1261 SCOPE 1.01 (0.99, 1.04) 0.2
Insulin (uU/ml) Active.B1271 / Active.B1261 STOP 1.01 (0.99, 1.04) 0.2
HOMA-IR Active.B1271 / Active.B1261 SCOPE 1.01 (0.99, 1.04) 0.2
HOMA-IR Active.B1271 / Active.B1261 STOP 1.01 (0.99, 1.04) 0.2
Glucose (mmol/L) Active.B1271 / Active.B1261 SCOPE 1.00 (1.00, 1.01) 0.3
Glucose (mmol/L) Active.B1271 / Active.B1261 STOP 1.00 (1.00, 1.01) 0.3

14.8.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.8.6 Plots